cell lines tb 1 lu atcc ccl Search Results


tb 1 lu cells  (ATCC)
95
ATCC tb 1 lu cells
Tb 1 Lu Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
tb 1 lu cells - by Bioz Stars, 2026-02
95/100 stars
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t.brasiliensis lung epithelial cell line (bat cell line)  (National Centre for Cell Science)
90
National Centre for Cell Science t.brasiliensis lung epithelial cell line (bat cell line)
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
T.Brasiliensis Lung Epithelial Cell Line (Bat Cell Line), supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t.brasiliensis lung epithelial cell line (bat cell line)/product/National Centre for Cell Science
Average 90 stars, based on 1 article reviews
t.brasiliensis lung epithelial cell line (bat cell line) - by Bioz Stars, 2026-02
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tb 1  (New England Biolabs)
86
New England Biolabs tb 1
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Tb 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tb 1/product/New England Biolabs
Average 86 stars, based on 1 article reviews
tb 1 - by Bioz Stars, 2026-02
86/100 stars
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glarine 100 iu/ml  (InsuLine Medical Ltd)
90
InsuLine Medical Ltd glarine 100 iu/ml
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Glarine 100 Iu/Ml, supplied by InsuLine Medical Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glarine 100 iu/ml/product/InsuLine Medical Ltd
Average 90 stars, based on 1 article reviews
glarine 100 iu/ml - by Bioz Stars, 2026-02
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gsmn-tb 1.1  (Biolog Inc)
90
Biolog Inc gsmn-tb 1.1
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Gsmn Tb 1.1, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gsmn-tb 1.1/product/Biolog Inc
Average 90 stars, based on 1 article reviews
gsmn-tb 1.1 - by Bioz Stars, 2026-02
90/100 stars
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pcdna3-tb 1  (Thermo Fisher)
90
Thermo Fisher pcdna3-tb 1
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Pcdna3 Tb 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
pcdna3-tb 1 - by Bioz Stars, 2026-02
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trio-thermoblock tb-1  (Biometra)
90
Biometra trio-thermoblock tb-1
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Trio Thermoblock Tb 1, supplied by Biometra, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trio-thermoblock tb-1/product/Biometra
Average 90 stars, based on 1 article reviews
trio-thermoblock tb-1 - by Bioz Stars, 2026-02
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turbidimeter tb 1  (VELP Scientifica)
90
VELP Scientifica turbidimeter tb 1
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Turbidimeter Tb 1, supplied by VELP Scientifica, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
turbidimeter tb 1 - by Bioz Stars, 2026-02
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trio tb-1 thermocycler  (Biometra)
90
Biometra trio tb-1 thermocycler
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Trio Tb 1 Thermocycler, supplied by Biometra, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
trio tb-1 thermocycler - by Bioz Stars, 2026-02
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technibryte micro tb-1 c tester  (Technidyne)
90
Technidyne technibryte micro tb-1 c tester
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Technibryte Micro Tb 1 C Tester, supplied by Technidyne, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
technibryte micro tb-1 c tester - by Bioz Stars, 2026-02
90/100 stars
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coating unit tb-1  (Kaikai Yuansheng Medicine Co Ltd)
90
Kaikai Yuansheng Medicine Co Ltd coating unit tb-1
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Coating Unit Tb 1, supplied by Kaikai Yuansheng Medicine Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/coating unit tb-1/product/Kaikai Yuansheng Medicine Co Ltd
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coating unit tb-1 - by Bioz Stars, 2026-02
90/100 stars
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mdr tb  (Eppendorf AG)
90
Eppendorf AG mdr tb
Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of <t>Tb1</t> Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.
Mdr Tb, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.

Journal: iScience

Article Title: Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling

doi: 10.1016/j.isci.2024.111444

Figure Lengend Snippet: Nsp13 promotes RNA-binding channel-dependent bat cell death, and CoV-RHIM-1 function is less critical (A) Microscopic analysis of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B, Nsp13- Δ1B-Rec1A-Rec2A and EGFP followed by puromycin treatment. Scale bar, 50μm. (B) Immunoblot analysis of lysates from Tb1 Lu cells showing expression of Nsp13-WT and its mutants after lentivirus transduction. (C) Representative Caspase-3/7 and Sytox green staining images of Tb1 Lu cells infected with lentiviruses expressing SARS-CoV-2 Nsp13-WT, Nsp13-Tet-mut, Nsp13-Δ1B and Nsp13- Δ1B-Rec1A-Rec2A followed by puromycin treatment acquired by Incucyte imaging analysis system. Scale bar, 200μm. (D) Real-time cell death measurement by Caspase-3/7 and Sytox green staining of Tb1 Lu cells infected as in panel-C. ∗∗∗∗ p < 0.0001, ∗∗∗ p = 0.0003, ∗∗ p = 0.0046 (Sytox green staining), ∗∗ p = 0.0086 (Caspase-3/7 staining), ns, not significant (two-way ANOVA, n = 3). Data shown are mean ± SEM. (E and F) Immunoblot analysis of RIPK1, RIPK3, ZBP1 and MLKL in lysates from `Tb1Lu cells after mock treatment, Nsp13 or Poly(I:C) transfection. The antibodies used for detecting these proteins were specific to human and mouse proteins. (G and H) Real-time cell death measurement by Sytox green staining of Tb1 Lu (G) and HT-29 cells (H), after treatment of TNF, zVAD and SMACmimetic (SMACmim). ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3), ns – not significant (two-way ANOVA). (I and J) Real-time cell death measurement by Sytox green staining of Tb1 Lu cells transfected with Poly(I:C) (I) or treated with Curaxin (J) ∗∗∗∗ p < 0.0001 (two-way ANOVA, n = 3). Data shown are mean ± SEM. (K) Immunoblot analysis of phosphorylated MLKL (pMLKL) in lysates from Tb1Lu cells after mock or Nsp13 transfection.

Article Snippet: Tb1 Lu ( T.brasiliensis lung epithelial cell line (bat cell line)) , National Centre for Cell Science (NCCS) cell repository , N/A.

Techniques: RNA Binding Assay, Infection, Expressing, Western Blot, Transduction, Staining, Imaging, Transfection

Journal: iScience

Article Title: Bat RNA viruses employ viral RHIMs orchestrating species-specific cell death programs linked to Z-RNA sensing and ZBP1-RIPK3 signaling

doi: 10.1016/j.isci.2024.111444

Figure Lengend Snippet:

Article Snippet: Tb1 Lu ( T.brasiliensis lung epithelial cell line (bat cell line)) , National Centre for Cell Science (NCCS) cell repository , N/A.

Techniques: Virus, Recombinant, Transfection, Staining, Western Blot, Electron Microscopy, Plasmid Preparation, Software, Microscopy